CIPF Centro de Investigacion Principe Felipe 2 articles published in JoVE Medicine A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice Fátima Manzano-Núñez1, Ruby Peters2, Deborah J. Burks1,3, Luke A. Noon1,3 1Centro de Investigación Príncipe Felipe (CIPF), 2Department of Physiology, Development and Neuroscience, University of Cambridge, 3Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM) We present a robust, cost-effective, and flexible method for measuring changes in hepatocyte number and nuclear ploidy within fixed/cryopreserved tissue samples that does not require flow cytometry. Our approach provides a powerful sample-wide signature of liver cytology ideal for tracking the progression of liver injury and disease. Biology Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis Jessica Martinez1, Viviana Bisbal2, Nerea Marin2, Antonio Cano1,3,4, Raul Gómez1 1Instituto de Investigación Sanitaria, 2Unidad de Animalario del Centro de Investigación Príncipe Felipe (CIPF), 3Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, 4Servicio de Obstetricia y Ginecología, Hospital Clínico Universitario de Valencia Here, we present a protocol for live-imaging of fluorescently labeled human endometrial fragments grafted in mice. The method allows studying the effects of drugs of choice on endometriotic lesion size through monitoring and quantification of fluorescence emitted by the fluorescent reporter on real time
