Spanish National Research Council (CSIC) 2 articles published in JoVE Neuroscience Transient Middle Cerebral Artery Occlusion Model of Stroke Nerea Chaparro-Cabanillas1, Maria Arbaizar-Rovirosa1, Angélica Salas-Perdomo1, Mattia Gallizioli1, Anna M. Planas1, Carles Justicia1 1Department of Neuroscience and Experimental Therapeutics, Institute for Biomedical Research of Barcelona (IIBB), Spanish National Research Council (CSIC) This protocol describes the model of transient focal cerebral ischemia in mice through intraluminal occlusion of the middle cerebral artery. Additionally, examples of outcome assessment are shown using magnetic resonance imaging and behavioral tests. Environment Long-term Video Tracking of Cohoused Aquatic Animals: A Case Study of the Daily Locomotor Activity of the Norway Lobster (Nephrops norvegicus) Jose A. Garcia*1,2, Valerio Sbragaglia*3,4, David Masip1, Jacopo Aguzzi2 1Universitat Oberta de Catalunya, 2Institute of Marine Sciences, Spanish National Research Council (CSIC), 3Institute for Environmental Protection and Research (ISPRA), 4Department of Biology and Ecology of Fishes, Leibniz-Institute of Freshwater Ecology and Inland Fisheries Here we present a protocol to individually track animals over a long period of time. It uses computer vision methods to identify a set of manually constructed tags by using a group of lobsters as case study, simultaneously providing information on how to house, manipulate, and mark the lobsters.
