CEU San Pablo University 2 articles published in JoVE Biochemistry Cryo-EM and Single-Particle Analysis with Scipion A. Jiménez-Moreno1, L. del Caño1, M. Martínez1, E. Ramírez-Aportela1, A. Cuervo1, R. Melero1, R. Sánchez-García1, D. Strelak1,2,3, E. Fernández-Giménez1, F.P. de Isidro-Gómez1, D. Herreros1, P. Conesa1, Y. Fonseca1, D. Maluenda1, J. Jiménez de la Morena1, J.R. Macías1, P. Losana1, R. Marabini1, J.M. Carazo1, C.O.S. Sorzano1,4 1Centro Nacional de Biotecnología, Campus Universidad Autónoma de Madrid, 2Faculty of Informatics, Masaryk University, 3Institute of Computer Science, Masaryk University, 4Campus Urbanización Montepríncipe, Universidad San Pablo CEU Single-particle analysis in cryo-electron microscopy is one of the main techniques used to determine the structure of biological ensembles at high resolution. Scipion provides the tools to create the whole pipeline to process the information acquired by the microscope and achieve a 3D reconstruction of the biological specimen. Immunology and Infection Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy Carlos Oscar S. Sorzano*1,2, Laura Martínez-Muñoz*3,4, Graciela Cascio3,5, Eva M. García-Cuesta3, J. Vargas1,6, Mario Mellado3, Jose Miguel Rodriguez Frade3 1Department of Macromolecular Structures, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n, Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology, McGill Univ. Here, we present a protocol for single particle tracking image analysis that allows quantitative evaluation of diffusion coefficients, types of motion and cluster sizes of single particles detected by fluorescence microscopy.
