the Biomedical Research Network in Cancer (CIBERONC)

1 article published in JoVE

• Cancer Research

  
  A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications Julie Earl¹, Silvia Calabuig-Fariñas¹, María Eugenia Sarasquete¹, Laura Muinelo Romay¹, Sara Lopez-Tarruella¹, Beatriz Bellosillo Paricio¹, Marta Rodríguez¹, Karmele Valencia Leoz¹, Marta Dueñas Porto¹, Noelia Tarazona¹, Javier Hernandez Losa¹, Rodrigo Almeida Toledo^{1 1}The Liquid Biopsy and Biomarker Working Module, the Biomedical Research Network in Cancer (CIBERONC) The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research laboratories.