3 articles published in JoVE
AFM and Microrheology in the Zebrafish Embryo Yolk Cell Maria Marsal1, Ignasi Jorba2, Elena Rebollo1, Tomas Luque2, Daniel Navajas2, Enrique Martín-Blanco1 1Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, 2Institute for Bioengineering of Catalonia, Universitat de Barcelona and CIBER Enfermedades Respiratorias The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow Mireia Ferrer*1, Lorena Martin-Jaular*1, Maria Calvo2, Hernando A. del Portillo1,3 1Department of poverty related diseases, Barcelona Centre for International Health Research, 2Confocal Microscopy Unit, University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA) We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses Sílvia Bofill-Mas1, Ayalkibet Hundesa1, Byron Calgua1, Marta Rusiñol1, Carlos Maluquer de Motes1, Rosina Girones1 1Laboratory of Water and Food Viral Pollution, Department of Microbiology, Faculty of Biology, University of Barcelona The study describes a cost-effective method for the identification of the source of fecal/urine contamination or contamination by nitrates in water using qPCR for the specific quantification of human/porcine/bovine DNA viruses, adenoviruses and polyomaviruses, proposed as MST tools.