Hopital Europeen Georges Pompidou 3 articles published in JoVE Cancer Research Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells Clémence Granier1, Emeline Vinatier2,3,4, Elia Colin2,3, Marion Mandavit2,3, Charles Dariane2,3,5, Virginie Verkarre6, Lucie Biard7, Rami El Zein2, Corinne Lesaffre2, Isabelle Galy-Fauroux2, Hélène Roussel2,3,6, Eléonore De Guillebon8, Charlotte Blanc2,3, Antonin Saldmann4, Cécile Badoual2,6, Alain Gey*4, Éric Tartour*2,4 1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou Studying tumor microenvironment may identify prognostic or predictive biomarkers of clinical response to immunotherapy. Presented here, is an innovative method based on in situ fluorescence multispectral imaging to analyze and count automatically various subpopulations of CD8+ T cells. This reproducible and reliable technique is suitable for large cohort analyses. Medicine ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection Guillaume Perrod1,2,3, Laetitia Pidial3, Sophie Camilleri1,4, Alexandre Bellucci1,3,5, Amaury Casanova3, Thomas Viel3, Bertrand Tavitian1,3,5, Chirstophe Cellier1,2, Olivier Clément1,3,5, Gabriel Rahmi1,2,3 1Assistance Publique-Hôpitaux de Paris, Université Paris Descartes Sorbonne Paris Cité, 2Department of Gastroenterology, Hôpital Européen Georges Pompidou, 3UMR-S970, Université Paris Descartes Sorbonne Paris Cité, 4Department of Pathology, Hôpital Européen Georges Pompidou, 5Department of Radiology, Hôpital Européen Georges Pompidou This study reports a successful method of endoscopical adipose tissue-derived stromal cell (ADSC)-sheet transplantation for esophageal stricture prevention after an extended endoscopic submucosal dissection (ESD) in a swine model. Medicine In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy Victor Fitoussi1, Nathalie Faye1, Foucauld Chamming's1,2, Olivier Clement1,2, Charles-Andre Cuenod1,2, Laure S. Fournier1,2 1Université Paris Descartes Sorbonne Paris Cité, INSERM UMR-S970, 2Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Service de Radiologie In this paper, we present a method to analyze tumor microvessels in vivo using dynamic contrast-enhanced fluorescence videomicroscopy. Two quantitative parameters were acquired: functional capillary density reflecting the vascularity of the tumor, and index leakage reflecting the leakiness of the endothelial wall.
