University Hospital of Clermont-Ferrand 3 articles published in JoVE Medicine Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation Carmen Mariana Aanei1, Marie-Christine Jacob2, Richard Veyrat-Masson3, Tiphanie Picot1, Maria Alessandra Rosenthal-Allieri4, Anne-Catherine Lhoumeau5, Michel Ticchioni4, Florent Dumezy6, Lydia Campos Catafal1 1Department of Hematology, University Hospital of Saint-Etienne, 2Department of Immunology, University Hospital of Grenoble-Alpes, 3Department Hematology, University Hospital of Clermont-Ferrand, 4Department of Immunology, University Hospital of Nice, 5Department of Biopathology, Institute Paoli-Calmettes, 6Department of Hematology, University Hospital of Lille The MDS diagnosis is difficult in the absence of morphological criteria or non-informative cytogenetics. MFC could help refine the MDS diagnostic process. To become useful for clinical practice, the MFC analysis must be based on parameters with sufficient specificity and sensitivity, and data should be reproducible between different operators. Medicine In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells Raïko Blondonnet1,2, Bertille Paquette1,2, Damien Richard3, Rémi Bourg2,4, Géraldine Laplace2,4, Romain Segurel2,4, Henria Pouvelle2,4, Corinne Belville2, Loic Blanchon2, Thomas Godet1,2, Jean-Michel Constantin1,2, Jean-Etienne Bazin1,2, Vincent Sapin2, Matthieu Jabaudon1,2 1Department of Perioperative Medicine, CHU Clermont-Ferrand, 2Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD), Université Clermont Auvergne, 3Department of Pharmacology, CHU Clermont-Ferrand, 4Nurse Anesthetist School, CHU Clermont-Ferrand We describe an easy protocol specifically designed to reach precise and controlled concentrations of sevoflurane or isoflurane in vitro in order to improve our understanding of mechanisms involved in the epithelial lung injury and to test novel therapies for acute respiratory distress syndrome. Biology TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos Benjamin Bertin1, Yoan Renaud1, Rajaguru Aradhya2, Krzysztof Jagla1, Guillaume Junion1 1University of Clermont-Ferrand, 2Memorial Sloan Kettering Cancer Center Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.
