3 articles published in JoVE
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues Imen Jebeniani1, Julia Leschik2, Michel Puceat1 1INSERM UMR-910, Faculté de Médecine, Université Aix-Marseille, 2Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg University A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.
Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics Sreekanth Dasari1, Pascal Weber1, Camélia Makhloufi1, Erica Lopez2, Claire-Lise Forestier1 1INSERM 1095, URMITE, University of Aix-Marseille, 2U.F.R de Médecine, University of Aix-Marseille This article reports on a detailed method for the dynamic measurement and quantification of blood flow velocity within individual blood vessels of the mouse liver vasculature using intravital microscopy imaging in combination with a specific methodology for image acquisition and analysis.
Cell Labeling and Injection in Developing Embryonic Mouse Hearts Emilye Hiriart*1, Patrick van Vliet*2, Ralf J. Dirschinger2, Sylvia M. Evans2, Michel Puceat1 1INSERM UMR-910, Aix-Marseille University, 2Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.