Universite Paris Diderot, Sorbonne Paris Cite 2 articles published in JoVE Neuroscience Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress Olivier Etienne1,2,3,4, Amandine Bery1,2,3,4, Telma Roque1,2,3,4, Chantal Desmaze1,2,3,4, François D. Boussin1,2,3,4 1Laboratoire de Radiopathologie, CEA DSV iRCM SCSR, 2INSERM, U967, 3Université Paris Diderot, Sorbonne Paris Cité, 4Université Paris Sud, UMR 967 Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development. Immunology and Infection A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions Mandy Muller*1,2, Patricia Cassonnet*1, Michel Favre1, Yves Jacob1,3, Caroline Demeret1 1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH), Institut Pasteur, 2Cellule Pasteur, Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology, Dana Farber Cancer Institute This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA.
