Ecole Normale Superieure de Lyon

3 articles published in JoVE

• Developmental Biology

  
  A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse Carla Guzman-Moreno*¹, Peizhao Zhang*², Olivia R. Phillips*¹, Mathias Block*³, Benjamin J. Glennon*¹, Meghan Holbrook², Lauren Weigand², Cecilia W. Lo¹, Jiuann-Huey I. Lin^{1,4 1}Department of Developmental Biology, University of Pittsburgh, ²Department of Biological Sciences, University of Pittsburgh, ³Département de Biologie, École Normale Supérieure de Lyon, ⁴Department of Critical Care Medicine, University of Pittsburgh This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.

• Genetics

  
  Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae Diedre Reitz¹, Jérôme Savocco², Aurèle Piazza², Wolf-Dietrich Heyer^{1,3 1}Department of Microbiology & Molecular Genetics, University of California, Davis, ²Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, ³Department of Molecular & Cellular Biology, University of California, Davis The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.

• Biology

  
  The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells Pierre Dourlen^1,2, Clemence Levet¹, Alexandre Mejat¹, Alexis Gambis³, Bertrand Mollereau^{1 1}Laboratory of Molecular Biology of the Cell, Ecole Normale Supérieure de Lyon, ²INSERM U744, Institut Pasteur de Lille, Université Lille-Nord de France, ³Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer, The Rockefeller University The Tomato/GFP-FLP/FRT method involves visualizing mosaic photoreceptor cells in living Drosophila. It can be used to follow individual photoreceptor cell fates in the retina for days or weeks. This method is ideal for studies of retinal degeneration and neurodegenerative diseases or photoreceptor cell development.