CNRS/Institut de Biologie Moleculaire et Cellulaire 2 articles published in JoVE Biochemistry Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip Raphaël de Wijn1,4, Kévin Rollet1,2, Vincent Olieric3, Oliver Hennig2, Nicola Thome1, Camille Noûs1, Caroline Paulus1, Bernard Lorber1, Heike Betat2, Mario Mörl2, Claude Sauter1 1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature. Bioengineering Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry Iris Marangon*1, Nicole Boggetto*2, Cécilia Ménard-Moyon3, Nathalie Luciani1, Claire Wilhelm1, Alberto Bianco3, Florence Gazeau1 1Laboratoire Matière et Systèmes Complexes (MSC), CNRS/Université Paris Diderot, 2ImagoSeine BioImaging Core Facility, Institut Jacques Monod, CNRS/Université Paris Diderot, 3Laboratoire d'Immunopathologie et Chimie Thérapeutique, CNRS/Institut de Biologie Moléculaire et Cellulaire In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.
