INRA ENVA ANSES 1 article published in JoVE Immunology and Infection Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors Marion Szelechowski1, Corinne Bergeron2, Daniel Gonzalez-Dunia1, Bernard Klonjkowski2 1INSERM UMR 1043, CNRS UMR 5282, Université Toulouse 3, 2UMR Viroligie, INRA ENVA ANSES These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.
