Centre Hospitalier Universitaire des Alpes 1 article published in JoVE Biochemistry Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment Magali Court1,2, Marie Malier1,2, Arnaud Millet1,2,3 1Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, 2Université Grenoble Alpes, 3Pôle Recherche, Centre Hospitalier Universitaire des Alpes We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.
