2 articles published in JoVE
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans Ana Soriano-Arroquia1, Peter D. Clegg1, Andrew P. Molloy1,2, Katarzyna Goljanek-Whysall1 1Institute of Ageing and Chronic Disease, University of Liverpool, 2Aintree University Hospital This protocol describes a robust, reproducible and simple method of isolation and culture of myoblast progenitor cells from the skeletal muscle of adult and aged people. The muscles used here include foot and leg muscles. This approach enables the isolation of an enriched population of primary myoblasts for functional studies.
Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield Andrea M. Collins1,2, Jamie Rylance3, Daniel G. Wootton4, Angela D. Wright3,5, Adam K. A. Wright1,3, Duncan G. Fullerton3,6, Stephen B. Gordon3 1Biomedical Research Centre in Microbial Diseases, National Institute for Health Research, 2Respiratory Infection Group, Royal Liverpool and Broadgreen University Hospital Trust, 3Respiratory Infection Group, Liverpool School of Tropical Medicine, 4Institute of Infection and Global Health, University of Liverpool, 5Comprehensive Local Research Network, Royal Liverpool and Broadgreen University Hospital Trust, 6Department of Respiratory Research, University Hospital Aintree We describe a research technique for fiberoptic bronchoscopy and bronchoalveolar lavage using low pressure suction. The technique is used to harvest immune cells from the lung bronchoalveolar surfaces. Local anesthetic and mild conscious sedation (midazolam) is used. Subjects tolerate the procedure well and experience minimal side effects.