2 articles published in JoVE
Optimization of a Quantitative Micro-neutralization Assay Yipu Lin1, Yan Gu1, John W. McCauley1 1Mill Hill Laboratory, The Francis Crick Institute This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites Erik Serrao1, Peter Cherepanov2, Alan N. Engelman1 1Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 2Chromatin Structure and Mobile DNA, The Francis Crick Institute We describe a protocol for amplifying retroviral integration sites from the genomic DNA of infected cells, sequencing the amplified virus-host junctions, and then mapping these sequences to a reference genome. We also describe techniques to quantify the distribution of integration sites relative to various genomic annotations using BEDTools.