Wellcome Sanger Institute, Wellcome Genome Campus 2 articles published in JoVE Genetics Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens Sumana Sharma1,2, Gavin J. Wright1 1Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, 2EMBL-EBI, Wellcome Genome Campus This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions. Genetics Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient Theresa Feltwell1, Matthew J. Dorman1, David A. Goulding1, Julian Parkhill1, Francesca L. Short1,2 1Wellcome Sanger Institute, Wellcome Genome Campus, 2Department of Medicine, University of Cambridge We demonstrate the use of discontinuous density gradients to separate bacterial populations based on capsule production. This method is used to compare capsule amount between cultures, isolate mutants with a specific capsule phenotype, or to identify capsule regulators. Described here is the optimization and running of the assay.
