MSD R&D Innovation Centre

1 article published in JoVE

• Neuroscience

  
  Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche Thomas Kerloch¹, Tjaša Lepko², Kirill Shkura², François Guillemot¹, Sébastien Gillotin^{2 1}The Francis Crick Institute, ²MSD R&D Innovation Centre Presented here is a method to sequence single nuclei isolated from the mouse dentate gyrus that excludes most neurons through fluorescence-activated nuclei (FAN)-sorting. This approach generates high-quality expression profiles and facilitates the study of most other cell types represented in the niche, including scarce populations such as neural stem cells.