Tel-Aviv University 10 articles published in JoVE Bioengineering Controlled Strain of 3D Hydrogels under Live Microscopy Imaging Avraham Kolel1, Avishy Roitblat Riba2, Sari Natan3, Oren Tchaicheeyan3, Eilom Saias2, Ayelet Lesman3,4 1Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering, Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems, Tel-Aviv University The presented method involves uniaxial stretching of 3D soft hydrogels embedded in silicone rubber while allowing live confocal microscopy. Characterization of the external and internal hydrogel strains as well as fiber alignment are demonstrated. The device and protocol developed can assess the response of cells to various strain regimes. Engineering Measurements of Local Instantaneous Convective Heat Transfer in a Pipe - Single and Two-phase Flow Adam Fershtman1, Dvora Barnea1, Lev Shemer1 1School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University This manuscript describes methods aimed at measuring the local instantaneous convective heat transfer coefficients in a single or two-phase pipe flow. A simple optical method to determine the length and the propagation velocity of an elongated (Taylor) air bubble moving at a constant velocity is also presented. Immunology and Infection Selective Harvesting of Marginating-hepatic Leukocytes Liat Sorski*1, Lee Shaashua*1, Rivka Melamed1, Pini Matzner1, Shamgar Ben-Eliyahu1 1Sagol School of Neuroscience & School of Psychological Sciences, Tel-Aviv University Marginating-hepatic leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe a method for selective harvesting of this specific hepatic cell population, through forced perfusion of the liver of rats or mice. Marginating-hepatic leukocytes seem critical in determining susceptibility to hepatic-related diseases and metastases. Behavior The Emotional Stroop Task: Assessing Cognitive Performance under Exposure to Emotional Content Moshe Shay Ben-Haim1, Paul Williams2, Zachary Howard2, Yaniv Mama3, Ami Eidels2, Daniel Algom1 1School of Psychological Sciences, Tel-Aviv University, 2School of Psychology, University of Newcastle, 3Department of Behavioral Sciences, Ariel University The emotional Stroop effect (ESE) is the result of longer naming latencies to ink colors of emotion words than those of neutral words. This report refers to potential sources of confounding and includes a modal experiment that provides the means to control for them. Immunology and Infection Selective Harvesting of Marginating-pulmonary Leukocytes Lee Shaashua*1, Liat Sorski*1, Rivka Melamed1, Shamgar Ben-Eliyahu1 1Sagol School of Neuroscience & School of Psychological Sciences, Tel-Aviv University Marginating-pulmonary leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe selective harvesting of this subpopulation of pulmonary leukocytes, by forced perfusion of the lungs in rats and mice. Marginating-pulmonary leukocytes seem critical in determining susceptibility to blood-borne and lung-related diseases. Neuroscience Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits Sivan Kanner*1, Marta Bisio*2, Gilad Cohen3, Miri Goldin4, Marieteresa Tedesco5, Yael Hanein3, Eshel Ben-Jacob4, Ari Barzilai1, Michela Chiappalone2, Paolo Bonifazi1,4 1Department of Neurobiology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, 2Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 3School of Electrical Engineering, Tel-Aviv University, 4School of Physics and Astronomy, Tel-Aviv University, 5Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genova This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity. Behavior The Forced Swim Test as a Model of Depressive-like Behavior Roni Yankelevitch-Yahav1,2, Motty Franko2, Avrham Huly2, Ravid Doron2,3,4 1School of Psychological Sciences, Tel-Aviv University, 2School of Behavioral Sciences, Academic College of Tel Aviv-Yaffo, 3Department of Education and Psychology, The Open University of Israel, 4School of Health and Life Sciences, Hadassah Academic College This protocol describes the forced swim test, which is used for the study of depressive-like behavior in rodents. This procedure involves placing an animal in a container filled with water that eventually will lead to the exhibition of immobility behavior, which is considered to reflect behavioral despair. Behavior Signal Attenuation as a Rat Model of Obsessive Compulsive Disorder Koral Goltseker*1, Roni Yankelevitch-Yahav*1, Noa S. Albelda*1, Daphna Joel1,2 1School of Psychological Sciences, Tel-Aviv University, 2Sagol School of Neuroscience, Tel-Aviv University The goal of the protocol described in this paper is to induce compulsive-like behavior in rats for the study of obsessive-compulsive disorder (OCD). This behavior is precipitated by attenuating a signal indicating that a lever-press response was effective in producing food. Neuroscience Extracting Visual Evoked Potentials from EEG Data Recorded During fMRI-guided Transcranial Magnetic Stimulation Boaz Sadeh1, Galit Yovel1,2 1School of Psychological Sciences, Tel-Aviv University, 2Sagol School of Neuroscience, Tel-Aviv University This paper describes a method for collecting and analyzing electroencephalography (EEG) data during concurrent transcranial magnetic stimulation (TMS) guided by activations revealed with functional magnetic resonance imaging (fMRI). A method for TMS artifact removal and extraction of event related potentials is described as well as considerations in paradigm design and experimental setup. Medicine Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells Asher Castiel1, Leonid Visochek2,3, Leonid Mittelman4, Yael Zilberstein4, Francoise Dantzer5, Shai Izraeli1,6, Malka Cohen-Armon2,3 1Cancer Research Center, Sheba Medical Center, 2The Neufeld Cardiac Research Institute, Tel-Aviv University, 3Department of Physiology and Pharmacology, Tel-Aviv University, 4Imaging Unit, Sackler Faculty of Medicine, Tel-Aviv University, 5Biotechnology and Cell Signaling, Ecole Superieure de Biotechnologie Strasbourg, 6Department of Human Molecular Genetics and Biochemistry, Tel-Aviv University The cytotoxic activity of the phenanthridine PJ-34 in cancer cells undergoing mitosis was documented in real time by live confocal imaging. PJ-34 eradicated human breast cancer MDA-MB-231 cells harboring extra-centrosomes in mitosis. Unlike normal bi-focal mitosis, the extra-centrosomes were not clustered in the two spindle poles in the presence of PJ-34.
