3 articles published in JoVE
Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses Fulvia Milena Cribiù*1, Claudia Burrello*2, Roberta Tacchi1, Francesca Boggio1, Dario Ricca1, Flavio Caprioli3,4, Stefano Ferrero1, Federica Facciotti2 1Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 2Department of Experimental Oncology, European Institute of Oncology, 3 Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin Monica Soldi1, Tiziana Bonaldi1 1Department of Experimental Oncology, European Institute of Oncology By combining native and crosslinking chromatin immunoprecipitation with high-resolution Mass Spectrometry, ChroP approach enables to dissect the composite proteomic architecture of histone modifications, variants and non-histonic proteins synergizing at functionally distinct chromatin domains.
A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants Katerina Tsilingiri1, Angelica Sonzogni2, Flavio Caprioli3, Maria Rescigno1 1Department of Experimental Oncology, European Institute of Oncology, 2Department of Pathology and Laboratory Medicine, European Institute of Oncology, 3U.O. Gastroenterologia 2, IRCCS Ca' Granda, Ospedale Policlinico di Milano We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.