IRCCS Neuromed 1 article published in JoVE Neuroscience Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease Alba Di Pardo1, Salvatore Castaldo1, Luca Capocci1, Enrico Amico1, Vittorio Maglione1 1Centre for Neurogenetics and Rare Diseases, IRCCS Neuromed In this study, we present an easy and efficient procedure for evaluating the disruption of the blood-brain barrier under neurodegenerative conditions. To achieve our objective, we infused high molecular weight fluorescein isothiocyanate labelled (FITC)-albumin into mouse jugular vein and evaluated its leakage into the brain parenchyma by fluorescence microscopy.
