2 articles published in JoVE
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma Alessandro De Vita1, Laura Mercatali1, Giacomo Miserocchi1, Chiara Liverani1, Chiara Spadazzi1, Federica Recine1, Alberto Bongiovanni1, Federica Pieri2, Davide Cavaliere3, Valentina Fausti1, Dino Amadori1, Toni Ibrahim1 1Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Pathology Unit, Morgagni-Pierantoni Hospital, 3Unit of Surgery and Advanced Oncologic Therapies, Morgagni-Pierantoni Hospital The following protocol focuses on the establishment of a primary culture of patient-derived soft tissue sarcoma (STS). This model could help us to better understand the molecular background and pharmacological profile of these rare malignancies and could represent a starting point for further research aimed at improving STS management.
Cell-Free DNA Integrity Analysis in Urine Samples Valentina Casadio1, Samanta Salvi1, Filippo Martignano1, Roberta Gunelli2, Sara Ravaioli1, Daniele Calistri1 1Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Department of Urology, Morgagni-Pierantoni Hospital A method for analyzing DNA integrity in the cell-free supernatant fraction of urine samples is proposed. The method is suitable for early detection of urological malignancies and has proven accurate for the early diagnosis of bladder cancer.