3 articles published in JoVE
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes Giacomo Siano1, Maria Claudia Caiazza1, Martina Varisco1, Mariantonietta Calvello1, Valentina Quercioli1, Antonino Cattaneo1, Cristina Di Primio1 1Laboratory of Biology, BIO@SNS, Scuola Normale Superiore Tau is a neuronal protein present both in the cytoplasm, where it binds microtubules, and in the nucleus, where it exerts unconventional functions including the modulation of Alzheimer's disease-related genes. Here, we describe a method to investigate the function of nuclear Tau while excluding any interferences coming from cytoplasmic Tau.
Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation Giulia Panattoni1,2, Lucia Rota1, Emanuela Colla1 1Bio@SNS Laboratory, Scuola Normale Superiore, 2International School for Advanced Studies (SISSA/ISAS) The goal of this protocol is to provide a cell-based system that replicates the formation of alpha-synuclein aggregates in vivo. Intracellular alpha-synuclein inclusions are seeded in primary neurons by the internalization and propagation of exogenous administered native microsomes-associated alpha-synuclein aggregates isolated from diseased alpha-synuclein transgenic mice.
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope Carmine Di Rienzo1,2, Enrico Gratton3, Fabio Beltram1, Francesco Cardarelli2 1NEST Laboratory, Scuola Normale Superiore, 2Center for Nanotechnology Innovation, Instituto Italiano di Tecnologia, 3Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.