International School for Advanced Studies (SISSA/ISAS) 4 articles published in JoVE Genetics Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies Simone Chiola1, Manuela Santo1, Antonello Mallamaci1 1Laboratory of Cerebral Cortex Development, SISSA Based on in vitro lentiviral engineering of neuronal precursors, their co-transplantation into wild-type brains and paired morphometric evaluation of "test" and "control" derivatives, this method allows accurate modeling of in vivo gene control of neocortical neuron morphology in a simple and affordable way. Neuroscience Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation Giulia Panattoni1,2, Lucia Rota1, Emanuela Colla1 1Bio@SNS Laboratory, Scuola Normale Superiore, 2International School for Advanced Studies (SISSA/ISAS) The goal of this protocol is to provide a cell-based system that replicates the formation of alpha-synuclein aggregates in vivo. Intracellular alpha-synuclein inclusions are seeded in primary neurons by the internalization and propagation of exogenous administered native microsomes-associated alpha-synuclein aggregates isolated from diseased alpha-synuclein transgenic mice. Neuroscience Preparation of Neuronal Co-cultures with Single Cell Precision Ngoc-Duy Dinh1, Ya-Yu Chiang1,2, Heike Hardelauf1, Sarah Waide1, Dirk Janasek1, Jonathan West1,3 1Leibniz-Institut für Analytische Wissenschaften, ISAS, 2Department of Biochemical Engineering, University College London, 3Institute for Life Sciences, University of Southampton Protocols for single neuron microfluidic arraying and water masking for the in-chip plasma patterning of biomaterial coatings are described. Highly interconnected co-cultures can be prepared using minimal cell inputs. Neuroscience Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis Josh Titlow1, Zana R. Majeed1,2, John G Nicholls3, Robin L. Cooper1 1Department of Biology, University of Kentucky, 2Department of Biology, College of Science, University of Salahaddin, Iraq, 3Department of Neurobiology and Cognitive Neuroscience, SISSA, Italy This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.
