University of Padua 4 articles published in JoVE Medicine A Rodent Model of The Ross Operation: Syngeneic Pulmonary Artery Graft Implantation in A Systemic Position Arben Dedja*1, Claudia Cattapan*1, Giovanni Di Salvo2, Martina Avesani2, Jolanda Sabatino2, Alvise Guariento1,3, Vladimiro Vida1 1Pediatric and Congenital Cardiac Surgery Unit, Department of Cardiac, Thoracic and Vascular Sciences and Public Health, University of Padua, 2Pediatric Cardiology Unit, Departments of Women’s and Children’s Health, University of Padua, 3Labatt Family Heart Centre, Department of Cardiovascular Surgery, The Hospital for Sick Children, University of Toronto We demonstrate how to establish a murine model of pulmonary root implantation into the descending aorta to simulate the Ross procedure. This model enables the medium/long-term evaluation of pulmonary autograft remodeling in a systemic position, representing the basis of developing therapeutic strategies to promote its adaptation. Genetics Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis Eleftheria Saplaoura*1, Valentina Perrera*2, Vincent Colot3, Friedrich Kragler1 1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3 The methylated RNA immunoprecipitation assay is an antibody-based method used to enrich for methylated RNA fragments. Coupled with deep sequencing, it leads to the identification of transcripts carrying the m5C modification. Behavior Comparative Lesions Analysis Through a Targeted Sequencing Approach Caterina Vicentini1, Andrea Mafficini1, Michele Simbolo2, Matteo Fassan3, Pietro Delfino2, Rita Teresa Lawlor1, Borislav Rusev1, Aldo Scarpa1,2, Vincenzo Corbo1,2 1ARC-Net Research Centre, University and Hospital Trust of Verona, 2Department of Diagnostics and Public Health, Section of Pathology, University and Hospital Trust of Verona, 3Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit, University of Padua This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors. Medicine Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model Gaia Codolo1, Fabio Munari1, Matteo Fassan2, Marina de Bernard1 1Department of Biology, University of Padua, 2Department of Medical Diagnostic Sciences & Special Therapies, University of Padua Here the method to establish a syngeneic mouse model of orthotopic bladder tumour to evaluate the anti-tumour efficacy of the bacterial protein HP-NAP is described.
