2 articles published in JoVE
Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives Roberta Maggiulli1, Alberto Vaiarelli1, Danilo Cimadomo1, Adriano Giancani1, Luisa Tacconi1, Gemma Fabozzi1, Filippo Maria Ubaldi1, Laura Rienzi1 1Clinica Valle Giulia, GeneraLife IVF Oocyte cryopreservation is recognized by several international scientific societies as the gold standard for fertility preservation in postpubertal women. Appropriate clinical and laboratory strategies ensure maximum efficacy, efficiency, and safety of fertility preservation treatments.
Human Blastocyst Biopsy and Vitrification Roberta Maggiulli1, Adriano Giancani1,2, Danilo Cimadomo1, Filippo M. Ubaldi1, Laura Rienzi1 1G.EN.E.R.A. Centers for Reproductive Medicine, 2DAHFMO, Unit of Histology and Medical Embryology, Sapienza University of Rome Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.