Universita Cattolica del Sacro Cuore 2 articles published in JoVE Immunology and Infection Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments Adele De Ninno1, Francesca Romana Bertani1, Annamaria Gerardino1, Giovanna Schiavoni2, Martina Musella3, Claudia Galassi3, Fabrizio Mattei2, Antonella Sistigu3,4, Luca Businaro1 1CNR Institute for Photonics and Nanotechnology, 2Dept. of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 3Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, 4Tumor Immunology and Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute In the age of immunotherapy and single-cell genomic profiling, cancer biology requires novel in vitro and computational tools for investigating the tumor-immune interface in a proper spatiotemporal context. We describe protocols to exploit tumor-immune microfluidic co-cultures in 2D and 3D settings, compatible with dynamic, multiparametric monitoring of cellular functions. Bioengineering Targeting the Rat's Small Bowel: Long-Term Infusion into the Superior Mesenteric Artery Alessandro Borrello1, Amedea Luciana Agnes1, Eleonora Pellegrino1, Sabina Magalini1, Daniele Gui1 1Department of Emergency Surgery, Università Cattolica del S. Cuore Access for long-term infusion in the superior mesenteric artery (SMA) of rats is a surgical procedure that consists of cannulation of a proximal branch of the SMA. The cannula exits from the abdominal wound and is tunneled through the subcutaneous space back to the interscapular fold.
