European Laboratory for Non Linear Spectroscopy 2 articles published in JoVE Biology Mesoscopic Optical Imaging of Whole Mouse Heart Francesco Giardini*1, Erica Lazzeri*1, Camilla Olianti*1, Giada Beconi1, Irene Costantini1,2, Ludovico Silvestri1,3,4, Elisabetta Cerbai1,5, Francesco S. Pavone1,3,4, Leonardo Sacconi1,3,6 1European Laboratory for Non-Linear Spectroscopy, 2Department of Biology, University of Florence, 3National Institute of Optics, National Research Council, 4Department of Physics and Astronomy, University of Florence, 5Department of Neurosciences, Psychology, Drugs and Child Health, University of Florence, 6Institute for Experimental Cardiovascular Medicine, Faculty of Medicine, University of Freiburg We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope. Biology Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy L. Gardini1,2, A. V. Kashchuk2,3, F. S. Pavone1,2,3, M. Capitanio2,3 1National Institute of Optics, National Research Council, 2LENS, European Laboratory for Non-Linear Spectroscopy, 3Physics Department, University of Florence Presented here is a comprehensive protocol to perform ultrafast force-clamp experiments on processive myosin-5 motors, which could be easily extended to the study of other classes of processive motors. The protocol details all the necessary steps, from the setup of the experimental apparatus to sample preparation, data acquisition and analysis.
