3 articles published in JoVE
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions Oriana Romano1,2, Ingrid Cifola3, Valentina Poletti4, Marco Severgnini3, Clelia Peano3, Gianluca De Bellis3, Fulvio Mavilio1,4, Annarita Miccio1,2,4,5 1Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, 3Institute for Biomedical Technologies, CNR, 4Généthon, 5Sorbonne Paris Cité - Université Paris Descartes Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.
Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development Chryssostomos Chatgilialoglu1, Carla Ferreri1, Annalisa Masi1, Michele Melchiorre1, Anna Sansone1, Michael A. Terzidis1, Armida Torreggiani1 1ISOF - Bio Free Radicals, Consiglio Nazionale delle Ricerche Radical-based biomimetic chemistry has been applied to building-up libraries necessary for biomarker development.
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons Anna Boccaccio1,2, Claudia Sagheddu1, Anna Menini1,3 1SISSA, International School for Advanced Studies, 2Istituto di Biofisica, Consiglio Nazionale delle Ricerche, 3SISSA Unit, Italian Institute of Technology Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.