Eindhoven University of Technology

17 articles published in JoVE

• Bioengineering

  
  A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue Hanneke Crielaard¹, Su Guvenir Torun¹, Tamar B. Wissing^1,2, Pablo de Miguel Muñoz^1,3, Gert-Jan Kremers⁴, Frank J. H. Gijsen^1,3, Kim Van Der Heiden^1,2, Ali C. Akyildiz^{1,3 1}Department of Biomedical Engineering, Erasmus Medical Center, ²Department of Biomedical Engineering, Eindhoven University of Technology, ³Department of Biomechanical Engineering, Delft University of Technology, ⁴Erasmus Optical Imaging Center, Erasmus Medical Center We have developed a mechano-imaging pipeline to study the heterogeneous structural and mechanical atherosclerotic plaque properties. This pipeline enables correlation of the local predominant angle and dispersion of collagen fiber orientation, the rupture behavior, and the strain fingerprints of the fibrous plaque tissue.

• Bioengineering

  
  Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates Cas van der Putten^1,2, Mirko D’Urso^1,2, Maaike Bril^1,2, Thomas E. Woud^1,2, Carlijn V. C. Bouten^1,2, Nicholas A. Kurniawan^{1,2 1}Department of Biomedical Engineering, Eindhoven University of Technology, ²Institute for Complex Molecular Systems, Eindhoven University of Technology Traditionally, cell culture is performed on planar substrates that poorly mimic the natural environment of cells in vivo. Here we describe a method to produce cell culture substrates with physiologically relevant curved geometries and micropatterned extracellular proteins, allowing systematic investigations into cellular sensing of these extracellular cues.

• Engineering

  
  A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch Suzanne E. Koch^1,2, Eline E. van Haaften^1,2, Tamar B. Wissing^1,2, Lizzy A. B. Cuypers¹, Jurgen A. Bulsink¹, Carlijn V. C. Bouten^1,2, Nicholas A. Kurniawan*^1,2, Anthal I. P. M. Smits*^{1,2 1}Department of Biomedical Engineering, Eindhoven University of Technology, ²Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology The goal of this protocol is to execute a dynamic co-culture of human macrophages and myofibroblasts in tubular electrospun scaffolds to investigate material-driven tissue regeneration, using a bioreactor which enables the decoupling of shear stress and cyclic stretch.

• Bioengineering

  
  A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression Ardjan J. van der Linden¹, Maaruthy Yelleswarapu², Pascal A. Pieters¹, Zoe Swank³, Wilhelm T. S. Huck², Sebastian J. Maerkl³, Tom F. A. de Greef^{1,2 1}Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, ²Institute for Molecules and Materials, Radboud University, ³Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL) The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.

• Engineering

  
  A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms Nidhi Sinha*¹, Nikita Subedi*¹, Florian Wimmers*², Melf Soennichsen¹, Jurjen Tel^{1 1}Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering, Eindhoven University of Technology, ²Institute for Immunity, Transplantation, and Infection, Beckman Center, Stanford University This article presents a protocol for seeding scarce population of cells using pipette-tips to droplet microfluidic devices in order to provide higher encapsulation efficiency of cells in droplets.

• Bioengineering

  
  Anatomically Realistic Neonatal Heart Model for Use in Neonatal Patient Simulators Mark Thielen¹, Frank Delbressine¹, Sidarto Bambang Oetomo^1,2, Loe Feijs^{1 1}Department of Industrial Design, Eindhoven University of Technology, ²Department of Neonatology, Máxima Medisch Centrum Veldhoven This protocol describes a procedure for creating functional artificial neonatal heart models by utilizing a combination of magnetic resonance imaging, 3D printing, and injection molding. The purpose of these models is for integration into the next generation of neonatal patient simulators and as a tool for physiological and anatomical studies.

• Bioengineering

  
  Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance Marinke W. van der Helm¹, Mathieu Odijk¹, Jean-Philippe Frimat^1,2, Andries D. van der Meer³, Jan C.T. Eijkel¹, Albert van den Berg¹, Loes I. Segerink^{1 1}BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics, University of Twente, ²Microsystems, Eindhoven University of Technology, ³Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally applicable.

• Engineering

  
  Preparation of Liquid Crystal Networks for Macroscopic Oscillatory Motion Induced by Light Ghislaine Vantomme*^1,2, Anne Helene Gelebart*^1,3, Dirk J. Broer^1,3, E. W. Meijer^{1,2 1}Institute for Complex Molecular Systems (ICMS), Technical University of Eindhoven, ²Department of Chemical Engineering and Chemistry, Laboratory of Macromolecular and Organic Chemistry, Technical University of Eindhoven, ³Department of Chemical Engineering and Chemistry, Laboratory for Functional Organic Materials and Devices (SFD), Technical University of Eindhoven The goal of the protocol is to create liquid crystalline polymer films that can mechanically oscillate under continuous light irradiation. We describe in great detail the conception of free-standing films, from the liquid crystal alignment method to the photo-actuation. The experimental protocol applied to prepare this material is broadly applicable.

• Chemistry

  
  Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry Dirk van den Bekerom¹, Niek den Harder¹, Teofil Minea¹, Nicola Gatti^1,2, Jose Palomares Linares¹, Waldo Bongers¹, Richard van de Sanden^1,3, Gerard van Rooij^{1,3 1}Dutch Institute for Fundamental Energy Research, ²University of Trento, ³Eindhoven University of Technology This article describes a flowing microwave reactor that is used to drive efficient non-equilibrium chemistry for the application of conversion/activation of stable molecules such as CO2, N2 and CH4. The goal of the procedure described here is to measure the in situ gas temperature and gas conversion.

• Chemistry

  
  Synthesis and Characterization of Supramolecular Colloids Neus Vilanova^1,4, Isja De Feijter^1,2,4, Ilja K. Voets^{1,3,4 1}Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, ²Laboratory of Chemical Biology, Eindhoven University of Technology, ³Laboratory of Physical Chemistry, Eindhoven University of Technology, ⁴Institute for Complex Molecular Systems, Eindhoven University of Technology A protocol for the synthesis and characterization of colloids coated with supramolecular moieties is described. These supramolecular colloids undergo self-assembly upon the activation of the hydrogen-bonds between the surface-anchored molecules by UV-light.

• Bioengineering

  
  Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor Sandra Van de Wiel¹, Maarten Merkx^2,3, Stan Van de Graaf^{1 1}Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, ²Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), ³Department of Biomedical Engineering, Eindhoven University of Technology We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.

• Engineering

  
  Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model İlker Doğan¹, Mauritius C. M. van de Sanden^{1,2 1}Department of Applied Physics, Eindhoven University of Technology, ²Dutch Institute for Fundamental Energy Research We demonstrate how to determine the size distribution of semiconductor nanocrystals in a quantitative manner using Raman spectroscopy employing an analytically defined multi-particle phonon confinement model. Results obtained are in excellent agreement with the other size analysis techniques like transmission electron microscopy and photoluminescence spectroscopy.

• Bioengineering

  
  An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart A. C. H. Pape*¹, Maarten H. Bakker*¹, Cheyenne C. S. Tseng², Maartje M. C. Bastings¹, Stefan Koudstaal², Pierfrancesco Agostoni², Steven A. J. Chamuleau², Patricia Y. W. Dankers^{1 1}Institute for Complex Molecular Systems, Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, ²Department of Cardiology, Division Heart and Lungs, Interuniversity Cardiology Institute of the Netherlands (ICIN), University Medical Center Utrecht Supramolecular hydrogelators based on ureido-pyrimidinones allow full control over the macroscopic gel properties and the sol–gel switching behavior using pH. Here, we present a protocol for formulating and injecting such a supramolecular hydrogelator via a catheter delivery system for local delivery directly in relevant areas in the pig heart.

• Bioengineering

  
  Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization Nicky de Jonge¹, Frank P. T. Baaijens¹, Carlijn V. C. Bouten^{1 1}Department of Biomedical Engineering, Eindhoven University of Technology This model system starts from a myofibroblast-populated fibrin gel that can be used to study endogenous collagen (re)organization real-time in a nondestructive manner. The model system is very tunable, as it can be used with different cell sources, medium additives, and can be adapted easily to specific needs.

• Bioengineering

  
  Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation Daisy W. J. van der Schaft¹, Ariane C. C. van Spreeuwel¹, Kristel J. M. Boonen¹, Marloes L. P. Langelaan¹, Carlijn V. C. Bouten¹, Frank P. T. Baaijens^{1 1}Department of Biomedical Engineering, Soft Tissue Biomechanics and Engineering, Eindhoven University of Technology, The Netherlands Engineered muscle tissue has great potential in regenerative medicine, as disease model and also as an alternative source for meat. Here we describe the engineering of a muscle construct, in this case from mouse myoblast progenitor cells, and the stimulation by electrical pulses.

• Engineering

  
  Controlling the Size, Shape and Stability of Supramolecular Polymers in Water Pol Besenius¹, Isja de Feijter², Nico A.J.M. Sommerdijk³, Paul H.H. Bomans³, Anja R. A. Palmans^{2 1}Organic Chemistry Institute and CeNTech, Westfälische Wilhelms-Universität Münster, ²Laboratory of Macromolecular and Organic Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology, ³Laboratory of Materials and Interface Chemistry and Soft Matter Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology The goal of this experiment is to determine and control the size, shape and stability of self-assembled discotic amphiphiles in water. For aqueous based supramolecular polymers such level of control is very difficult. We apply a strategy using both repulsive and attractive interactions. The experimental techniques applied to characterize this system are broadly applicable.

• Medicine

  
  Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice Michael T. Kuhlmann¹, Simon Cuhlmann^2,3, Irmgard Hoppe¹, Rob Krams³, Paul C. Evans², Gustav J. Strijkers⁴, Klaas Nicolay⁴, Sven Hermann¹, Michael Schäfers^{1 1}European Institute for Molecular Imaging, Westfälische Wilhelms-University Münster, ²British Heart Foundation Cardiovascular Sciences Unit, Imperial College London, ³Department of Bioengineering, Imperial College London, ⁴Biomedical Engineering, Eindhoven University of Technology The constricting cuff presented in this article is designed to induce atherosclerosis in the murine common carotid artery. Due to the conical shape of its inner lumen the implanted cuff generates well-defined regions of low, high and oscillatory shear stress triggering the development of atherosclerotic lesions of different inflammatory phenotypes.