Institute of Complex Molecular Systems 1 article published in JoVE Bioengineering Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor Sandra Van de Wiel1, Maarten Merkx2,3, Stan Van de Graaf1 1Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, 2Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), 3Department of Biomedical Engineering, Eindhoven University of Technology We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.
