Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht 2 articles published in JoVE Developmental Biology A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis Marek J. van Oostrom*1, Wilke H. M. Meijer*1, Katharina F. Sonnen1 1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis. Biology Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids Ravian L. van Ineveld*1,2,3, Hendrikus C.R. Ariese*1,2,3, Ellen J. Wehrens1,2,3, Johanna F. Dekkers*1,2,3,4, Anne C. Rios*1,2,3 1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.
