Norwegian University of Science and Technology (NTNU) 5 articles published in JoVE Bioengineering Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability Charissa Poon1,2, Melina Mühlenpfordt*3, Marieke Olsman*3, Spiros Kotopoulis4,5, Catharina de Lange Davies3, Kullervo Hynynen1,2,6 1Physical Sciences Platform, Sunnybrook Research Institute, 2Institute of Biomedical Engineering, University of Toronto, 3Department of Physics, Norwegian University of Science and Technology, 4Department of Clinical Medicine, University of Bergen, 5Exact Therapeutics AS, 6Department of Medical Biophysics, University of Toronto This protocol describes the surgical and technical procedures that enable real-time in vivo multiphoton fluorescence imaging of the rodent brain during focused ultrasound and microbubble treatments to increase blood-brain barrier permeability. Bioengineering Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release Charlotte Nawijn1, Tim Segers1,2, Guillaume Lajoinie1, Ýrr Mørch3, Sigrid Berg4,5,6, Sofie Snipstad3,6,7, Catharina de Lange Davies7, Michel Versluis1 1Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 2BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 3Department of Biotechnology and Nanomedicine, SINTEF Industry, 4Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, 5Department of Health Research, SINTEF Digital, 6Cancer Clinic, St. Olav’s Hospital, 7Department of Physics, Norwegian University of Science and Technology The presented protocols can be used to characterize the response of fluorescently-labeled microbubbles designed for ultrasound-triggered drug delivery applications, including their activation mechanisms as well as their bioeffects. This paper covers a range of in vitro and in vivo microscopy techniques performed to capture the relevant length and timescales. Neuroscience Event Related Potentials (ERPs) and other EEG Based Methods for Extracting Biomarkers of Brain Dysfunction: Examples from Pediatric Attention Deficit/Hyperactivity Disorder (ADHD) Geir Ogrim1,2,3, Juri D. Kropotov4,5 1Neuropsychiatric Team, Åsebråten Outpatient Clinic, Østfold Hospital Trust, 2Institute of Psychology, Norwegian University of Science and Technology, 3Gillberg Neuropsychiatry Centre, University of Gothenburg, 4P. Bechtereva Institute of the Human Brain, Russian Academy of Sciences, 5Department of Neuropsychology, Andrzej Frycz-Modrzewski Krakow University EEG-methods are applied for extracting biomarkers of brain dysfunctions. The focus is on multi-channel event-related potentials (ERPs) recorded in a cued GO/NOGO task. Non-brain artifacts are corrected and ERPs are compared with the normative data. Examples relate to biomarkers for ADHD diagnosis and prediction of medication response. Neuroscience Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains Giulia Quattrocolo1, Maria Isaac2, Yajun Zhang2, Timothy J. Petros2 1Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health Challenging young neurons in new brain regions can reveal important insights into how the environment sculpts neuronal fate and maturation. This protocol describes a procedure to harvest interneuron precursors from specific brain regions and transplant them either homotopically or heterotopically into the brain of postnatal pups. Environment Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge Simon Felz1, Salah Al-Zuhairy1, Olav Andreas Aarstad2, Mark C.M. van Loosdrecht1, Yue Mei Lin1 1Department of Biotechnology, Delft University of Technology, 2Department of Biotechnology, Norwegian Biopolymer Laboratory (NOBIPOL), Norwegian University of Science and Technology The protocol provides a methodology to solubilize aerobic granular sludge in order to extract alginate-like extracellular polymers (ALE).
