2 articles published in JoVE
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains Giulia Quattrocolo1, Maria Isaac2, Yajun Zhang2, Timothy J. Petros2 1Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health Challenging young neurons in new brain regions can reveal important insights into how the environment sculpts neuronal fate and maturation. This protocol describes a procedure to harvest interneuron precursors from specific brain regions and transplant them either homotopically or heterotopically into the brain of postnatal pups.
Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge Simon Felz1, Salah Al-Zuhairy1, Olav Andreas Aarstad2, Mark C.M. van Loosdrecht1, Yue Mei Lin1 1Department of Biotechnology, Delft University of Technology, 2Department of Biotechnology, Norwegian Biopolymer Laboratory (NOBIPOL), Norwegian University of Science and Technology The protocol provides a methodology to solubilize aerobic granular sludge in order to extract alginate-like extracellular polymers (ALE).