St. Olav Hospital 1 article published in JoVE Bioengineering Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release Charlotte Nawijn1, Tim Segers1,2, Guillaume Lajoinie1, Ýrr Mørch3, Sigrid Berg4,5,6, Sofie Snipstad3,6,7, Catharina de Lange Davies7, Michel Versluis1 1Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 2BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 3Department of Biotechnology and Nanomedicine, SINTEF Industry, 4Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, 5Department of Health Research, SINTEF Digital, 6Cancer Clinic, St. Olav’s Hospital, 7Department of Physics, Norwegian University of Science and Technology The presented protocols can be used to characterize the response of fluorescently-labeled microbubbles designed for ultrasound-triggered drug delivery applications, including their activation mechanisms as well as their bioeffects. This paper covers a range of in vitro and in vivo microscopy techniques performed to capture the relevant length and timescales.
