2 articles published in JoVE
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules Anaïs Aulas*1,2, Marta M. Fay*1,2, Witold Szaflarski1,2,3, Nancy Kedersha1,2, Paul Anderson1,2, Pavel Ivanov1,2,4 1 Stress Granules (SGs) are nonmembranous cytoplasmic structures that form in cells exposed to a variety of stresses. SGs contain mRNAs, RNA-binding proteins, small ribosomal subunits, translation-related factors, and various cell signaling proteins. This protocol describes a workflow that uses several experimental approaches to detect, characterize, and quantify bona fide SGs.
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts Urszula Polak1,2, Calley Hirsch1, Sherman Ku3, Joel Gottesfeld3, Sharon Y.R. Dent1, Marek Napierala1 1Department of Molecular Carcinogenesis and Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center, 2Department of Cell Biology, Poznan University of Medical Sciences, 3Department of Molecular Biology, The Scripps Research Institute We present a protocol for efficient reprogramming of human somatic cells into human induced pluripotent stem cells (hiPSC) using retroviral vectors encoding Oct3/4, Sox2, Klf4 and c-myc (OSKM) and identification of correctly reprogrammed hiPSC by live staining with Tra-1-81 antibody.