Centro Hospitalar de Lisboa Central 2 articles published in JoVE Medicine Functional and Physiological Methods of Evaluating Median Nerve Regeneration in the Rat Diogo Casal*1,2, Eduarda Mota-Silva*3, Inês Iria4,5, Diogo Pais1, Ana Farinho6, Sara Alves7, Cláudia Pen7, Luís Mascarenhas-Lemos7, José Ferreira-Silva7, Mário Ferraz-Oliveira7, Valentina Vassilenko3, Paula A. Videira4,5, João Goyri-O'Neill1 1Anatomy Department, NOVA Medical School, 2Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central, 3Physics Department, Faculty of Sciences and Technology, LIBPhys, 4Glycoimmunology, CEDOC, NOVA Medical School, 5UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 6Tissue Repair Unit, CEDOC, NOVA Medical School, 7Pathology Department, Centro Hospitalar de Lisboa Central Presented is a protocol to produce different types of median nerve (MN) lesions and repair in the rat. Additionally, the protocol shows how to evaluate the functional recovery of the nerve using several noninvasive behavioral tests and physiological measurements. Medicine A Model of Free Tissue Transfer: The Rat Epigastric Free Flap Diogo Casal1,4, Diogo Pais1, Inês Iria3,4, Eduarda Mota-Silva5, Maria-Angélica Almeida2, Sara Alves6, Cláudia Pen6, Ana Farinho4, Luís Mascarenhas-Lemos1,6, José Ferreira-Silva6, Mário Ferraz-Oliveira6, Valentina Vassilenko5, Paula A. Videira3,4, João Gory O'Neill1,5 1Anatomy Department, NOVA Medical School, Universidade NOVA de Lisboa, 2Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central - Hospital de São José, 3UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 4CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, 5Physics Department, Faculty of Sciences and Technology, LIBPhys, 6Pathology Department, Centro Hospitalar de Lisboa Central – Hospital de São José This paper describes the steps required to raise a fasciocutaneous epigastric free flap and transfer it to the neck in the rat.