Institute of Cellular Biology and Pathology 1 article published in JoVE Bioengineering An Efficient Method for Adenovirus Production Madalina Dumitrescu1, Violeta Georgeta Trusca1, Ioana Madalina Fenyo1, Anca Violeta Gafencu1 1Institute of Cellular Biology and Pathology "Nicolae Simionescu" Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.
