3 articles published in JoVE
Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival Per Wessman1, Sebastian Håkansson1, Klaus Leifer2, Stefano Rubino2 1Department of Microbiology, Uppsala Biocenter, Swedish University of Agricultural Sciences, 2Department of Engineering Sciences, Uppsala University Freeze-drying is often an easy and convenient way to obtain dry products of viable bacterial cells. An issue of the process is cell survival. We detail here a procedure to investigate how cell survival during freeze-drying is influenced by the properties of the formulation used.
Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast Pernilla Bjerling1, Ida Olsson1,2, Xi'nan Meng1 1Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, University of Uppsala, 2Department of Microbiology, Swedish University of Agricultural Sciences The fission yeast, Schizosaccharomyces pombe, is a good model system to study basic cellular processes. Here we describe a method to perform quantitative live cell analysis of fission yeast. In this particular experiment we focus on organisation of the genome within the cell nucleus, but the method can also be used to study cytosolic factors.
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species Anna Karlgren1, Jenny Carlsson2, Niclas Gyllenstrand2, Ulf Lagercrantz1, Jens F. Sundström2 1Department of Evolutionary Functional Genomics, Evolutionary Biology Center, Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.