Ananda Devices 1 article published in JoVE Neuroscience Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection Margaret H. Magdesian1,2,3, Madeleine Anthonisen1, G. Monserratt Lopez-Ayon1, Xue Ying Chua1, Matthew Rigby1, Peter Grütter1 1Department of Physics, McGill University, 2Department of Neurology and Neurosurgery, Montreal Neurological Institute, 3Ananda Devices This procedure describes how to rapidly initiate, extend and connect neurites organized in microfluidic chambers using poly-D-lysine-coated beads fixed to micropipettes that guide neurite elongation.