Universite de Sherbrooke 4 articles published in JoVE Biochemistry MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria Noémie Mercier1, Karine Prévost2, Eric Massé2, Pascale Romby1, Isabelle Caldelari1, David Lalaouna1 1Université de Strasbourg, CNRS, 2Department of Biochemistry, Université de Sherbrooke MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria. Genetics Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames Marie A. Brunet1,2, Xavier Roucou1,2 1Department of Biochemistry, Université de Sherbrooke, 2PROTEO, Quebec Network for Research on Protein Function, Structure, and Engineering OpenProt is a freely accessible database that enforces a polycistronic model of eukaryotic genomes. Here, we present a protocol for the use of OpenProt databases when interrogating mass spectrometry datasets. Using OpenProt database for analysis of proteomic experiments allows for discovery of novel and previously undetectable proteins. Medicine Assessment of the Efficacy of An Osteopathic Treatment in Infants with Biomechanical Impairments to Suckling Juliette Herzhaft-Le Roy1, Marianne Xhignesse2, Isabelle Gaboury2 1Entraide Naturo-Lait, 2Faculté de médecine et des sciences de la santé, Université de Sherbrooke Osteopathy is an emerging field of clinical research. Here we present a protocol to assess the efficacy of an osteopathic intervention coupled with lactation consultation, in infants with biomechanical issues impeding breastfeeding. Genetics Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation Antoine Gaudreau-Lapierre*1, Daniel Garneau*1, Billel Djerir*1, Frédéric Coulombe1, Théo Morin1, Alexandre Marechal1 1Department of Biology, Université de Sherbrooke Studying DNA damage repair kinetics requires a system to induce lesions at defined sub-nuclear regions. We describe a method to create localized double-stranded breaks using a laser-scanning confocal microscope equipped with a 405 nm laser and provide automated procedures to quantify the dynamics of repair factors at these lesions.
