Universite de Moncton 1 article published in JoVE Biochemistry Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue Chloé J Simard*1, Guillaume Pelletier*1, Luc H Boudreau1, Etienne Hebert-Chatelain2, Nicolas Pichaud1 1Département de chimie et biochimie, Université de Moncton, 2Département de biologie, Université de Moncton In this paper, a method to measure oxygen consumption using high-resolution respirometry in permeabilized thoraxes of Drosophila is described. This technique requires a minimal amount of tissue compared to the classic mitochondrial isolation technique and the results obtained are more physiologically relevant.
