Hospital for Sick Children Research Institute 3 articles published in JoVE Bioengineering Blood Flow Imaging with Ultrafast Doppler Jerome Baranger1,2, Luc Mertens1,2, Olivier Villemain1,2,3 1Translational Medicine Department, The Hospital for Sick Children, PGCRL Research Institute, 2The Labatt Family Heart Centre, Department of Pediatric, The Hospital for Sick Children, University of Toronto, 3Medical Biophysics Department, University of Toronto This protocol shows how to apply ultrafast ultrasound Doppler imaging to quantify blood flows. After a 1 s long acquisition, the experimenter has access to a movie of the full field of view with axial velocity values for each pixel every ≈0.3 ms (depending on the ultrasound time of flight). Biochemistry Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques June Ereño-Orbea*1, Taylor Sicard*1,2, Hong Cui1, Indira Akula1, Jean-Philippe Julien1,2,3 1Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 2Department of Biochemistry, University of Toronto, 3Department of Immunology, University of Toronto We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography. Developmental Biology Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach Yun-Ui Bae1, Hoon-Ki Sung2,3, Jae-Ryong Kim1 1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, 2Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.
