Institut du cancer de Montreal 2 articles published in JoVE Immunology and Infection Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment Manuel Flores Molina1,2, Thomas Fabre1,2, Aurélie Cleret-Buhot1, Geneviève Soucy1,3, Liliane Meunier1,4, Mohamed N. Abdelnabi1,2, Nicolas Belforte1,5, Simon Turcotte1,4,6, Naglaa H. Shoukry1,4,7 1 Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays. Immunology and Infection Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation Antonio Layoun1, Macha Samba1, Manuela M. Santos1,2 1Centre de recherche du Centre hospitalier de l'Université de Montréal, Institut du cancer de Montréal, 2Département de Médecine, Université de Montréal We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.
