Toronto Western Research Institute 1 article published in JoVE Developmental Biology Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord Andrea Mothe1, Charles H. Tator1,2 1Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Center, 2Department of Surgery, Division of Neurosurgery, Toronto Western Hospital and University of Toronto The adult mammalian spinal cord contains neural stem/progenitor cells (NSPCs) that can be isolated and expanded in culture. This protocol describes the harvesting, isolation, culture, and passaging of NSPCs generated from the periventricular region of the adult spinal cord from the rat and from human organ transplant donors.
