Hospital du Sacre-Coeur de Montreal 4 articles published in JoVE Neuroscience A Novel and Translational Rat Model of Concussion Combining Force and Rotation with In Vivo Cerebral Microdialysis Ian O. Massé1, Luc Moquin2, Chloé Provost1, Samuel Guay1, Alain Gratton2, Louis De Beaumont1 1Research Center, Hôpital du Sacré-Cœur de Montréal, 2Research Center, Douglas Institute Neurotransmitter alteration is a mechanism of neural dysfunction that occurs after concussion and contributes to the sometimes-catastrophic long-term consequences. This rat model combines microdialysis, allowing in vivo neurotransmitter quantification, with a weight-drop technique exerting rapid acceleration and deceleration of the head and torso, an important factor of human craniocerebral trauma. Neuroscience Thoracic Spinal Cord Hemisection Surgery and Open-Field Locomotor Assessment in the Rat Andrew R. Brown1,2, Marina Martinez1,2,3 1Department of Neurosciences, Faculté de Médecine, Université de Montréal, 2Hôpital du Sacré-Cœur de Montréal, 3Groupe de Recherche sur le Système Nerveux Central (GRSNC), Université de Montréal The rat thoracic spinal hemisection is a valuable and reproducible model of unilateral spinal cord injury to investigate the neural mechanisms of locomotor recovery and treatment efficacy. This article includes a detailed step-by-step guide to perform the hemisection procedure and to assess locomotor performance in an open-field arena. Neuroscience Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction Kim Gilbert1, Roger Godbout1, Guy Rousseau1 1Centre de Recherche, Hospital du Sacre-Coeur de Montreal Myocardial infarction (MI) is not only followed by impaired cardiac function but also by apoptosis in the amygdala, a brain region involved in the behavioral consequences of MI. This protocol describes how to induce MI, collect amygdala tissue and measure caspase-3 activity, a marker of apoptosis, therein. Medicine Using Continuous Data Tracking Technology to Study Exercise Adherence in Pulmonary Rehabilitation Amanda K. Rizk1,2,3, Rima Wardini2,3, Emilie Chan-Thim1,2,3, Barbara Trutschnigg3, Amélie Forget3, Véronique Pepin2,3 1Individualized Program, Concordia University, 2Department of Exercise Science, Concordia University, 3Centre de Recherche, Axe Maladies Chroniques, Hôpital du Sacré-Coeur de Montréal Pulmonary rehabilitation is widely recognized in the management of respiratory diseases. A key component to successful pulmonary rehabilitation is adherence to the recommended exercise training. The purpose of the present protocol is to describe how continuous data tracking technology can be used to precisely measure adherence to a prescribed aerobic training intensity.
