Princess Margaret Cancer Centre 5 articles published in JoVE Immunology and Infection Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System Marieke de Korte1,2, Armand Keating1,2,4, Chen Wang1,3 1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture. Medicine Synthesis and Characterization of Multi-Modal Phase-Change Porphyrin Droplets Kimoon Yoo1,2, Alexander Dhaliwal1,2, Juan Chen1, Paul S. Sheeran3, Gang Zheng1,2 1Princess Margaret Cancer Centre, 2Department of Medical Biophysics, University of Toronto, 3Philips Healthcare In this protocol, methods for synthesizing and characterizing multi-modal phase-change porphyrin droplets are outlined. Medicine Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors Shawn Stapleton1,2,3, Daniel Mirmilshteyn2, Jinzi Zheng3,4, Christine Allen2,4,5, David A. Jaffray1,2,3,4,5,6 1Department of Medical Biophysics, University of Toronto, 2Leslie Dan Faculty of Pharmacy, University of Toronto, 3STTARR Innovation Centre, Princess Margaret Cancer Centre, 4Institute of Biomaterials and Biomedical Engineering, University of Toronto, 5Techna Institute, University Health Network, 6Radiation Medicine Program, Princess Margaret Cancer Centre The heterogeneous intra-tumoral accumulation of liposomes has been linked to an abnormal tumor microenvironment. Herein methods are presented to measure tumor microcirculation by perfusion imaging and elevated interstitial fluid pressure (IFP) using an image-guided robotic system. Measurements are compared to the intra-tumoral accumulation of liposomes, determined using volumetric micro-CT imaging. Immunology and Infection Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow Yonghong Chen*1, Azusa Maeda*1,2, Jiachuan Bu1, Ralph DaCosta1,2,3 1Princess Margaret Cancer Centre, 2Department of Medical Biophysics, University of Toronto, 3Techna Institute, University Health Network The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time. Medicine A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics Kelly Burrell1, Sameer Agnihotri1, Michael Leung2, Ralph DaCosta2, Richard Hill2, Gelareh Zadeh3 1Brain Tumor Research Centre, Hospital for Sick Children, Toronto Medical Discovery Tower, 2Ontario Cancer Institute, Princess Margaret Hospital, 3Neurosurgery, Toronto Western Hospital We describe a novel in vivo imaging technique that couples fluorescent chimeric mice with intracranial windows and high-resolution 2-photon microscopy. This imaging platform aids studies of dynamic changes in brain tissue and microvasculature, at a single-cell level, following pathological insults and is adaptable to assess intracranial drug delivery and distribution.
