Vaccine and Infectious Disease Organization-International Vaccine Centre 1 article published in JoVE Genetics Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq) Melissa B. Palmer1,2, Yejun Wang3, Aaron P. White1,2 1Vaccine and Infectious Disease Organization-International Vaccine Centre, 2Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, 3Department of Cell Biology and Genetics, Shenzhen University Health Science Center Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a method used to establish interactions between transcription factors and the genomic sequences they control. This protocol outlines techniques for performing ChIP-seq with bacterial biofilms, using Salmonella enterica serovar Typhimurium bacterial biofilm as an example.
