Terry Fox Laboratory, BC Cancer 1 article published in JoVE Biology Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models Georgios Stroulios*1, Martin Stahl*2, Fisal Elstone*2, Wing Chang1, Sharon Louis2, Allen Eaves2,3, Salvatore Simmini1, Ryan K. Conder2 1STEMCELL Technologies Ltd., 2STEMCELL Technologies Inc., 3Terry Fox Laboratory, BC Cancer Here we present two protocols that allow the modeling of intestinal apical-specific interactions. Organoid-derived intestinal monolayers and Air-Liquid Interface (ALI) cultures facilitate the generation of well-differentiated epithelia accessible from both luminal and basolateral sides, whereas polarity-inverted intestinal organoids expose their apical side and are amenable to high throughput assays.
