McGill University Faculty of Medicine and Health Sciences 1 article published in JoVE Medicine Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model Athar Shadmani1, Hala Shakib Dhowre1, Ozlem Ercal1, Xiang Qi Meng2, Albert Y. Wu1 1Department of Ophthalmology, Stanford University School of Medicine, 2McGill University Faculty of Medicine and Health Sciences This protocol describes a method to induce an accurate and reproducible corneal and limbal alkali injury in a mouse model. The protocol is advantageous as it allows for an evenly distributed injury to the highly curved mouse cornea and limbus.
