University of Sonora 2 articles published in JoVE Neuroscience Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9 Irina Sinakevitch1,2, Zev Kurtzman*2, Hyun G. Choi*2, David Arturo Ruiz Pardo3, Romain A. Dahan2, Nathaniel Klein1, Branimir Bugarija4, Erik Wendlandt4, Brian H. Smith2 1Department of Neuroscience, University of Arizona, 2School of Life Sciences, Arizona State University, 3Department of Scientific and Technologic Investigations, University of Sonora, 4Integrated DNA Technologies, Inc. Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity. Bioengineering A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies María Dolores Figueroa-Pizano1, Itziar Vélaz2, María Elisa Martínez-Barbosa1 1Department of Polymers and Materials Research, University of Sonora, 2Department of Chemistry, Faculty of Sciences, University of Navarra The freezing-thawing method is used to produce chitosan-poly(vinyl alcohol) hydrogels without crosslinking agents. For this method, it is important to consider the freezing conditions (temperature, number of cycles) and polymer ratio, which can affect the properties and applications of the obtained hydrogels.
