NASA Ames Research Center View Institution's Website 3 articles published in JoVE Behavior Collecting Sleep, Circadian, Fatigue, and Performance Data in Complex Operational Environments Lucia Arsintescu1, Kenji H. Kato2, Cassie J. Hilditch1, Kevin B. Gregory1, Erin Flynn-Evans3 1San Jose State University Research Foundation, 2ASRS Research and Technology Solutions, 3Fatigue Countermeasures Laboratory, NASA Ames Research Center Sleep loss and circadian misalignment contribute to numerous operational accidents and incidents. The effectiveness of countermeasures and work scheduling designs aimed at mitigating fatigue can be challenging to evaluate in operational environments. This manuscript summarizes an approach for collecting sleep, circadian, fatigue, and performance data in complex operational environments. Genetics Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform Afshin Beheshti1, Yasaman Shirazi-Fard2, Sungshin Choi1, Daniel Berrios3, Samrawit G. Gebre1, Jonathan M. Galazka2, Sylvain V. Costes2 1WYLE Labs, Space Biosciences Division, NASA Ames Research Center, 2Space Biosciences Division, NASA Ames Research Center, 3USRA, NASA Ames Research Center The NASA GeneLab platform provides unfettered access to precious omics data from biological spaceflight experiments. We describe how a typical mouse experiment is conducted in space and how data from such experiments can be accessed and analyzed. Bioengineering Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research Wan W. Amani Wan Salim1, Michael A. Zeitchek1, Andrew C. Hermann1, Antonio J. Ricco2, Ming Tan2, Florian Selch2, Erich Fleming2, Brad M. Bebout2, Mamoun M. Bader3, Aeraj ul Haque4, D. Marshall Porterfield5 1Department of Agricultural and Biological Engineering, Birck-Bindley Physiological Sensing Facility, Purdue University, 2NASA Ames Research Center, 3Department of Chemistry, Pennsylvania State University Hazleton, 4Cooley LLP, 5NASA Life and Physical Sciences, Human Exploration and Operations Mission Directorate, NASA Headquarters All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.